Guinea Pig Interleukin 2 ELISA Kit with High Spesificity and Seitivity
Cat.No E0069Gp
Standard Curve Range: 10ng/L - 2000ng/L
Seitivity: 5.68ng/L
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
*This product is for research use only, not for use in diagnosis procedures. It&quo;s highly recommend to read this itruction entirely before use.

Intended Use
This sandwich kit is for the accurate quantitative detection of Guinea Pig Interleukin 2 (also known as IL-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Assay Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Guinea Pig IL-2 antibody. IL-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Guinea Pig IL-2 Antibody is added and binds to IL-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Guinea Pig IL-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Reagent Provided

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000